
URINE HORMONE EXTRACTION ACTION
Every sample that you are trying to test for estrogens or extract estrogens from must be treated differently, whether it is a river sample or a urine sample. This particular method has been performed multiple times for urine samples. However, the final product is a COLLECTION of sex hormones, steroidal molecules in general. To isolate only estrogen would require a further step of purification, which needs to be further investigated. The setup is fairly simple and DIY. You need: glass bottle, silica gel (desiccant), cigarette filters, parafilm, methanol, water, and a urine sample. After eluting, allow the methanol to evaporate until what is left is a brown sticky substance. (Time can vary depending on your method of evaporation). Congrats, you have hormones -- technically pheromones, now that they're acting outside the body.
EVOLUTION OF A PROTOCOL


Experimentations in the Slovenian mountains... thanks PIF camp!

Final Protocol
MATERIALS




