URINE HORMONE EXTRACTION ACTION




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First prototypes during Interactivos'16?: Possible Worlds in Madrid, Spain with collaborators: Byron Rich, Gaia Leandra, Paula Pin, Amanda Padilla, and Carlos Gamez 


Experimentations in the Slovenian mountains... thanks PIF camp!


SPECIAL THANKS TO SPELA PETRIC FOR HER AMAZING CONSULTATIONS ON URINE EXTRACTION!





MATERIALS




Every sample that you are trying to test for estrogens or extract estrogens from must be treated differently, whether it is a river sample or a urine sample. This particular method has been performed multiple times for urine samples. However, the final product is a COLLECTION of sex hormones, steroidal molecules in general. To isolate only estrogen would require a further step of purification, which needs to be further investigated. The setup is fairly simple and DIY. You need: glass bottle, silica gel (desiccant), cigarette filters, parafilm, methanol, water, and a urine sample. After eluting, allow the methanol to evaporate until what is left is a brown sticky substance. (Time can vary depending on your method of evaporation). Congrats, you have hormones -- technically pheromones, now that they're acting outside the body.